A Second Ouabain-sensitive Sodium-dependent Adenosine Triphosphatase in Brain Microsomes
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چکیده
منابع مشابه
Sodium-potassium-activated adenosine triphosphatase of brain microsomes: modification of sodium inhibition by diphenylhydantoins.
Effects of diphenylhydantoins on (Na(+) + K(+))-ATPase activity in rat and cat brain microsomes were studied. 5,5-diphenylhydantoin (DPH) in concentrations of 5-20 mug ml(-1) produces an apparent stimulation of the rat brain (Na(+) + K(+))-activated ATPase of 55-65% in media containing 50 mM Na(+), 0.15 mM K(+), 3 mM Mg(++), and 3 mM ATP. No effects are found on the Mg-ATPase. At constant K(+) ...
متن کاملA cycle for ouabain inhibition of sodium- and potassium-dependent adenosine triphosphatase.
Physiological ligands of (sodium-potassium)-dependent adenosine triphosphatase influenced the sensitivity of the enzyme to the inhibitor, ouabain. Phosphorylation of the native enzyme is accelerated by sodium ions, while potassium ions accelerate dephosphorylation. Exposure of the phosphoenzyme to ouabain slowed its turnover and rendered it insensitive to potassium ion. This action of ouabain d...
متن کاملChanges in erythrocyte membrane ouabain-sensitive adenosine triphosphatase after renal transplantation.
1. Intracellular electrolytes, and erythrocyte membrane adenosine triphosphatase (ATPase) activity, was studied in twenty patients after renal transplantation. 2. The mean ouabain-sensitive ATPase activity in the erythrocyte membranes of the transplant patients was 122 nmol of inorganic phosphorus (Pi) h-1 mg of tissue-1 (SEM 14), compared with 62 nmol of Pi h-1 mg of tissue-1 (SEM 8) in a grou...
متن کاملDecreased ouabain-sensitive adenosine triphosphatase activity in the erythrocyte membrame of patients with chronic renal disease.
1. Ouabain-sensitive ATPase (adenosine triphosphatase) activity was measured in the erythrocyte membranes of twenty patients with chronic renal disease. Decreased activity was found in nineteen of the twenty patients. The average decrease was 38%. 2. In three patients erythrocyte sodium concentration exceeded 9.5 mmol/l of RBC and these patients had the most marked decreases in ouabain-sensitiv...
متن کاملDistribution of lens sodium-potassium-adenosine triphosphatase.
PURPOSE The specific activity of sodium-potassium-adenosine triphosphatase (Na-K-ATPase) in lens fiber cells is lower than the specific activity in lens epithelium. To test whether there is a reduction in the expression of Na-K-ATPase molecules in lens fibers, a Western blot technique was used. METHODS Membrane material was isolated from different regions of the rabbit lens. Na-K-ATPase (aden...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1969
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)63489-1